We propose DINOBot, a novel imitation learning framework for robot manipulation, which leverages the image-level and pixel-level capabilities of features extracted from Vision Transformers trained with DINO. When interacting with a novel object, DINOBot first uses these features to retrieve the most visually similar object experienced during human demonstrations, and then uses this object to align its end-effector with the novel object to enable effective interaction. Through a series of real-world experiments on everyday tasks, we show that exploiting both the image-level and pixel-level properties of vision foundation models enables unprecedented learning efficiency and generalisation. Videos and code are available at https://sites.google.com/view/dinobot/.